I wish to raise the following two questions about human Toll-like receptor 4 (hTLR4).
1. Can we clone cDNA(s) encoding as-yet-unidentified hTLR4-binding protein(s) using yeast two-hybrid screening?
2. Is the use of human placenta cDNA library appropriate for such screening?
You might find this posting reminiscent of “MD-2“, one of my previous postings.