Human chromosomal passenger complex (CPC) is composed of Aurora-B, inner centromere protein, and so forth. So I wish to raise the following question:
1. Have we already identified all components of human CPC?
If not, two more questions can be raised:
2. Should we perform yeast two-hybrid screening with human Aurora-B as bait?
3. Should we employ human thymus cDNA prey library for that screening?
From now on, I will use the auxiliary “should” carefully.