Ectodomains of two distinct isoforms of Drosophila DSCAM have been crystallized, which leads me to raise the following three questions:
1. Can we measure the homophilic binding force between full-length human DSCAM (hDSCAM) using AFM in solution?
2. Can we detect heterophilic binding of hDSCAM using yeast-two hybrid method?
3. What transports hDSCAM to the cell surface?
This paper has just reminded me of the importance of Drosophila system.
For a visual explanation of being homo-/heterophilic, see here.
It has just been reported that most of Drosophila DSCAM isoforms exhibit homophilic binding.
I have just noticed this paper.
Domains 1-8 of Drosophila DSCAM have been crystallized.