Putative cargo-binding domain of human kinesin superfamily protein 4 (KIF4) is slightly different from that of murine KIF4. And murine KIF4 binds to poly (ADP-ribose) polymerase-1 (PARP-1). So I wish to raise the following four questions:
1. What kind of cargo does human KIF4 transport?
2. What kind of protein mediates the binding of human KIF4 to its cargo?
3. Can we observe the movement of human KIF4 with or without its cargo using AFM in solution?
4. What kind of tissue/cell is appropriate for AFM observation?
I have just found this paper slightly surprising.