Restin/CLIP-170 is a member of +TIPs, and Rac effector IQGAP1 captures microtubule via CLIP-170.
So I wish to raise the following three questions:
1. Is Rap1A, upstream regulator of Rac, also involved in capturing of microtubule via human CLIP-170?
2. This work seems to need reevaluating. So, in human cells, some kind of motor protein targets CLIP-170 to the plus end of microtubule like S. cerevisiae and S. pombe cases?
3. If so, can we observe the transport of CLIP-170 along the microtubule using AFM in solution?
Truncated human CLIP-170-p150glued complex has been crystallized.
Dimerization model for main +TIPs has been proposed.
The EB1 homolog Mal3, the CLIP-170 homolog Tip1, and the kinesin Tea2 have been in vitro reconstituted.
Does human CLIP-170 undergo hydroxylation?