FANC’s

Fanconi anemia (FA) is a rare autosomal recessive disease, which, in many cases, eventually results in acute myelogenous leukemia (see, e.g., Lichtman, M.A. et al. eds. Williams Hematology 7th ed. McGraw-Hill Medical 2005, p423-424.), and known FA gene products include FANCA, FANCB, FANCC, BRCA2 (breast cancer type 2 susceptibility protein) /FANCD1, FANCD2, FANCE, FANCF, FANCG, FANCI, BRIP1/FANCJ, FANCL, FANCM, and PALB2 (partner and localizer of BRCA2) /FANCN.

So I wish to raise the following four questions:

1. Is each subunit in the protein complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, and FANCM (FA core complex) monomeric?

2. Is AFM in solution an appropriate method to address the question above?

3. Have we already identified all FANC members?

4. If not, can we determine the stoichiometry and assembly of the protein complex composed of FA core complex plus as-yet-unidentified FANC member(s) using AFM in solution?

PS

Since ubiquitinated FANCD2 associates with BRCA2/FANCD1, I will discuss proteins involved in the suppression of breast cancer later.

Addendum primum:

I have just noticed this paper.

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

w

Connecting to %s