Fanconi anemia (FA) is a rare autosomal recessive disease, which, in many cases, eventually results in acute myelogenous leukemia (see, e.g., Lichtman, M.A. et al. eds. Williams Hematology 7th ed. McGraw-Hill Medical 2005, p423-424.), and known FA gene products include FANCA, FANCB, FANCC, BRCA2 (breast cancer type 2 susceptibility protein) /FANCD1, FANCD2, FANCE, FANCF, FANCG, FANCI, BRIP1/FANCJ, FANCL, FANCM, and PALB2 (partner and localizer of BRCA2) /FANCN.
So I wish to raise the following four questions:
1. Is each subunit in the protein complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, and FANCM (FA core complex) monomeric?
2. Is AFM in solution an appropriate method to address the question above?
3. Have we already identified all FANC members?
4. If not, can we determine the stoichiometry and assembly of the protein complex composed of FA core complex plus as-yet-unidentified FANC member(s) using AFM in solution?
Since ubiquitinated FANCD2 associates with BRCA2/FANCD1, I will discuss proteins involved in the suppression of breast cancer later.
I have just noticed this paper.