Like TLR9, Saccharomyces cerevisiae Mid1 is located to both the cell membrane and ER. And its stoichiometry has remained elusive. (see here and here.) Assuming that human homolog of Mid1 exists and functions as a stretch-activated calcium channel, I wish to raise the following three questions:

1. How is putative human homolog’s tissue distribution?

2. Does its subcellular localization resemble Saccharomyces cerevisiae Mid1’s?

3. Can we determine its stoichiometry and further observe its gating cycle using AFM in solution?