Human interleukin-6 (hIL-6) binds to its receptor hIL-6 receptor (hIL-6R), and the resultant hIL-6-hIL-6R complex further binds to human gp130 (hgp130). However, the stoichiometry and assembly of the complex composed of the aforementioned three kinds of molecules have remained elusive, raising the following two questions:
So can we determine the stoichiometry and assembly of the protein complex composed of full-length hIL-6-hIL-6R-hgp130 using AFM in solution?
I have found this paper a bit surprising.
I have just noticed this paper.