Questions about another motor protein:
KIF1A was once reported to be monomeric. However, as mentioned recently in this review, the Vale lab cast some doubt on a report from the Hirokawa lab. (for other relevant reviews, see, e.g., here and here.) So I wish to raise the following five questions:
1. Can we determine the stoichiometry and velocity of full-length axonal transporter of synaptic vesicles (ATSV), human ortholog of KIF1A, using AFM in solution (at least in vitro and preferably in non-contact mode)?
2. Or ATSV undergoes monomer-dimer transition like its Caenorhabditis elegans ortholog UNC104?
3. If so, is such transition mediated by K-loop?
4. Does ATSV move along the human microtubule in a K-loop-dependent fashion?
On its putative receptor liprin-α:
5. Is this also the case with human liprin-α?
I have recently noticed a paper demonstrating that Drosophila melanogaster liprin-α is required for synaptic vesicle trafficking.
Now I wish to raise the sixth question: Does D. melanogaster liprin-α bind to DUnc104?
I have just noticed this model.
I wish to raise the seventh question: Can we visualize dimeric KIF1A using AFM in solution?
I wish to raise the eighth question: Does full-length murine KIF1A move like an inchworm?
The ninth question: Can we verify the ‘two-door system‘ using AFM in solution?
I have just noticed this paper.
Assuming that we can utilize ATSV/KIF1A bait construct(s), I wish to raise the tenth question:
Is it worth proceeding to perform yeast two-hybrid screening/assay?