ATSV/KIF1A

Questions about another motor protein:

KIF1A was once reported to be monomeric. However, as mentioned recently in this review, the Vale lab cast some doubt on a report from the Hirokawa lab. (for other relevant reviews, see, e.g., here and here.) So I wish to raise the following five questions:

1. Can we determine the stoichiometry and velocity of full-length axonal transporter of synaptic vesicles (ATSV), human ortholog of KIF1A, using AFM in solution (at least in vitro and preferably in non-contact mode)?

2. Or ATSV undergoes monomer-dimer transition like its Caenorhabditis elegans ortholog UNC104?

3. If so, is such transition mediated by K-loop?

4. Does ATSV move along the human microtubule in a K-loop-dependent fashion?

On its putative receptor liprin-α:

Rat liprin-α seems to function as a KIF1A receptor.

5. Is this also the case with human liprin-α?

Addendum primum:

I have recently noticed a paper demonstrating that Drosophila melanogaster liprin-α is required for synaptic vesicle trafficking.

Addendum secundum:

Now I wish to raise the sixth question: Does D. melanogaster liprin-α bind to DUnc104?

Addendum tertium:

I have just noticed this model.

Addendum quartum:

I wish to raise the seventh question: Can we visualize dimeric KIF1A using AFM in solution?

Addendum quintum:

I wish to raise the eighth question: Does full-length murine KIF1A move like an inchworm?

Addendum sextum:

The ninth question: Can we verify the ‘two-door system‘ using AFM in solution?

Addendum septimum:

I have just noticed this paper.

Addendum octavum:

Assuming that we can utilize ATSV/KIF1A bait construct(s), I wish to raise the tenth question:

Is it worth proceeding to perform yeast two-hybrid screening/assay?

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