Whereas conventional kinesin is a heterotetramer composed of two heavy chains (KHC’s) and two light chains (KLC’s) and involved in intracellular cargo transport, Eg5 is a homotetramer, which is involved in the establishment of the bipolar spindleand inhibited by monastrol analogues. Although I recently found a study on dimeric Eg5 to be very impressive, I have believed that results based on the use of green-fluorescent protein (GFP) or artificial beads do not always reflect its real movement, considering the molecular weight of fused GFP and absence of such beads in living cells.
So can we observe how full-length human tetrameric Eg5 establishes bipolar spindle using AFM in solution (preferably in non-contact mode)?
Does human KIFC2 bind to human Eg5?
This minireview might help you answer the above question.
I have fixed the link pointing to “ATSV/KIF1A”.